Siberian Mouse M 45l ((TOP))
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Figure 3: (a) Level of the antibodies to rESAT6 antigen in the blood serum of the mice immunized with different antigens: 14 = 5.14 and P = 0.039 and "F114 = 12.05 and P = 0.0037; (b) level of the antibodies to rCFP10-ESAT6-dIFN with different methods of antigen presentation, blue bars, antigen delivered by injection, brown bars, antigen delivered orally: *F17 = 6.55 and P = 0.038 and "*F174 = 276.10 and P < 0.001 both when compared with the control for the mouse groups immunized with the corresponding antigen (repeated-measures ANOVA, two gradations of control as a factor and one of the antigens).
animals (F334 = 12.39, P < 0.001 for log-transformed data). Compared with controls, immunization with rCFP10-ESAT6-dIFN elevated the level of antibodies to rESAT6 in mouse blood serum, whereas immunization with rCFP10 decreased this level (Figure 3(a)).
The rCFP10-ESAT6-dIFN injections induced the maximal increase in the antibodies specific for this antigen in the mouse blood serum (Figure 3(b)). It should be emphasized that level of the antibodies to rCFP10-ESAT6-dIFN strikingly exceeded the control level (FL7 = 276.10 and P < 0.001). The levels of antibodies to rCFP10 and rESAT6 in mice immunized with rCFP10-ESAT6-dIFN were at the control level (Figure 3(b)).
Figure 4: The SI for the proliferation of peripheral blood mononuclear cells induced by recombinant proteins after immunization with different antigens. (a) The SI for rESAT6: "'Fl 14 = 15.36 and P = 0.0015 and "Fl 14 = 12.21 and P = 0.0036; (b) the SI for rCFP10: "'Fl 14 = 32.58 and P = 0.001 and "F1 14 = 11.63 and P = 0.0046; (c) the SI for rCFP10-ESAT6-dIFN. All F1A4 as compared to the control for the mouse group immunized with this antigen (repeated-measures ANOVA; administration route as a factor, two gradations of control as a factor, and one of the antigens); *P < 0.05 as compared with unity (Student's i-test).
Figure 5: The SI for the proliferation ofperipheral blood mononuclear cells induced by nonspecific proliferation inducers after immunization with different antigens: (a) the SI for ConA: "'F1 14 = 40.74 and P = 0.001 and "F1 14 = 15.85 and P = 0.0013; (b) the SI for PHA: '"F1 14 = 13.87 and P = 0.002, "F114 = 8.97 and P = 0.001, and 'F114 = 6.21 and P = 0.026. Both FU4 as compared to the control for the mouse group immunized with this antigen (repeated-measure ANOVA; administration route as a factor, two gradations of control as a factor, and one of the antigens); *P < 0.05 as compared with unity (Student's i-test).
[23]E. Yakushenko, J. Lopatnikova, E. Khrapov et al., "Use of transgenic carrot plants producing human interleukin-18 for modulation of mouse immune response," in New Research on Biotechnology in Biology and Medicine, A. M. Egorov and G. Zaikov, Eds., chapter 10, pp. 97-107, Nova Science Publishers, New York, NY, USA, 2006. 2b1af7f3a8